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Thiadiazoles prevent α ‐hemoysin mediated Ca 2+ influx in U937 monocytes. U937 cells were loaded with Fluo‐4 dye and treated with the indicated compound concentration. Hla (0.5 µg/mL) was added and Ca 2+ influx was determined after 4 h. EC 50 of selected analogs 1 (A), 6 (B), and 5 (C) as well as the inactive analog 7 lacking the basic nitrogen atom at the side chain (D) are shown. Data points indicate means ± SD, n = 3.

Journal: Chemmedchem

Article Title: An Imidazo[2,1‐b][1,3,4]thiadiazole Derivative Inhibits the Virulence Factor α‐Hemolysin by Blocking the Pullout of Its Stem Domain

doi: 10.1002/cmdc.202501098

Figure Lengend Snippet: Thiadiazoles prevent α ‐hemoysin mediated Ca 2+ influx in U937 monocytes. U937 cells were loaded with Fluo‐4 dye and treated with the indicated compound concentration. Hla (0.5 µg/mL) was added and Ca 2+ influx was determined after 4 h. EC 50 of selected analogs 1 (A), 6 (B), and 5 (C) as well as the inactive analog 7 lacking the basic nitrogen atom at the side chain (D) are shown. Data points indicate means ± SD, n = 3.

Article Snippet: U937 cells , DSMZ , ACC5.

Techniques: Concentration Assay